Reduced Representation Bisulfite Sequencing (RRBS)
RRBS is an efficient and high-throughput technique used to analyze the genome-wide methylation profiles on a single nucleotide level. As a result of the high cost and depth of sequencing needed to analyze methylation status in the entire genome, RRBS is a bisulfite-based protocol that enriches CG-rich parts of the genome, thereby reducing the amount of sequencing required while capturing the majority of promoters and other relevant genomic regions.
- Library prep based on optimized protocol from Messner et al.*
- Numerous customer publications based on results from RRBS-related research projects
* Messner et al. Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profiling. Nature Protocols 2011; 6:468–481.
How IntegraGen Genomics adds value
- Expertise in the design of research projects
- Optimized RRBS library prep protocol for human and mouse genomes
- Single Msp1 digestion or enhanced protocol (eRRBS) with double enzymatic digestions
- RRoxBS expertise available
- Ability to develop personalized tertiary statistical analyses of genomic data via our GeCo Advanced Genomic Consulting Service.
- High conversion rate (>99.5%)
- Sequencing depth and coverage guarantee according to project design
Analysis of RRBS and Methyl-Seq data:
- Primary analysis using BS-Seeker gold standard pipeline
- Standard delivery includes methylation rate per Cytosine in all sequence context
- Personalized tertiary statistical analyses via our GeCo Advanced Genomic Consulting Service.