Reduced Representation Bisulfite Sequencing (RRBS)
RRBS is an efficient and high-throughput technique used to analyze the genome-wide methylation profiles on a single nucleotide level. As a result of the high cost and depth of sequencing needed to analyze methylation status in the entire genome, RRBS is a bisulfite-based protocol that enriches CG-rich parts of the genome, thereby reducing the amount of sequencing required while capturing the majority of promoters and other relevant genomic regions.
- Library prep based on optimized protocol from Messner et al.*
- Numerous customer publications based on results from RRBS-related research projects
* Messner et al. Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profiling. Nature Protocols 2011; 6:468–481.
How IntegraGen Genomics adds value
- Expertise in the design of research projects
- Optimized RRBS library prep protocol for human and mouse genomes
- Single Msp1 digestion or enhanced protocol (eRRBS) with double enzymatic digestions
- RRoxBS expertise available
- Ability to develop personalized tertiary statistical analyses of genomic data via our GeCo Advanced Genomic Consulting Service.
- High conversion rate (>99.5%)
- Sequencing depth and coverage guarantee according to project design
Agilent SureSelectXT Human Methyl-Seq
Agilent SureSelectXT Human Methyl-Seq is the first comprehensive target enrichment system to enable researchers to focus on the regions where methylation is known to impact gene regulation. It targets the most complete content for methylation sequencing, including cancer tissue-specific DMRs. It has a 84Mb design content with coverage of 3.7 million individual CpG dinucleotides.
Analysis of RRBS and Methyl-Seq data:
- Primary analysis using BS-Seeker gold standard pipeline
- Standard delivery includes methylation rate per Cytosine in all sequence context
- Personalized tertiary statistical analyses via our GeCo Advanced Genomic Consulting Service.